The Michaelis constant of enzyme reaction at present condition was calculated. 计算了该实验条件下酶反应的米氏常数。
The apparent Michaelis constant and reaction velocity of the enzyme did not change after treatment with antibody. It was shown that the antibody might combine with a non-active site of acetylcholinesterase without affecting the conformation of its active site. 酶与抗体反应前后的表观米氏常数Km及反应速度没有明显变化,表明抗体是结合在酶的非活性部位,没有或很少影响酶的活性中心的构象。
The glutamate decarboxylase of the immobilized cells exhibited an increase in the apparent Michaelis constant ( K'm), optimum pH, pH stability. Study and Characteristics of Glutamate Decarboxylase Electrode 其中固定化细胞最适pH和pH稳定性增加,最适温度及热稳定性下降;L-谷氨酸脱羧酶电极的研制与性能
As a denaturant for protein, SDS not only makes the HRP lose its activity because of denaturalization, but also decrease the Michaelis constant and the maximum velocity of the reaction for the substrate OPD. SDS作为一种蛋白质变性剂,不仅能够使HRP变性失去部分活性,而且还能使HRP对底物OPD的米氏常数Km(OPD)和最大反应速率都比其在缓冲体系中小;
Determination of the Michaelis Constant of Single Substrate Enzyme catalysed Reaction by the First order Reaction Network Theory 一级反应网络法求单底物酶促反应的米氏常数
The Michaelis constant Km of β-amylase in upland rice variety is higher than in rice ones. 旱稻品种β-淀粉酶的Km值大于水稻。
The characteristics of the enzyme such as pH stabilization, heat stabilization and Michaelis constant were studied. 并对该酶的pH稳定性、热稳定性、米氏常数等进行了研究。
By substrate depletion approach, the apparent enzyme kinetics parameters michaelis constant ( Km), maximum velocity ( Vmax), and the intrinsic clearance ( CLint) were determined in rat liver microsomes. 利用底物消除法,测定了该类药物在大鼠肝微粒体中的表观酶动力学参数:米氏常数Km、最大反应速率Vmax和固有清除率CLint。